noaa-fisheries-afsc-data-socrata/afscracesapshavey-dna-extraction-from-archived-px7a-nf6c

  • alaska
  • bering sea
  • biota
  • blood smear
  • canada
  • + 7

AFSC/RACE/SAP/Shavey: DNA extraction from archived Giemsa-stained blood smears using polymerase chain reaction to detect host and parasitic DNA

AFSC/RACE/SAP/Shavey: DNA extraction from archived Giemsa-stained blood smears using polymerase chain reaction to detect host and parasitic DNA

These are the data from a laboratory experiment in which DNA content was removed from blood smears and extracted. The blood smears were either stained with Giemsa-stain or remained un-stained. Some of the un-stained smears were coverslipped. Host and parasitic DNA was amplified using conventional PCR.

Columns

NameSocrata field nameColumn name in sgr mountData typeDescription
16S_PRIMER_16s_primer_16s_primerNumberPrimer (0 = Did not use primer set on this sample, 1 = Used primer set on this sample)
SMEAR_RESULTsmear_resultsmear_resultNumberSmear result (0 = Hematodinium parasite absent on smear, 1 = Hematodinium parasite present on smear)
COI_PRIMERcoi_primercoi_primerNumberPrimer (0 = Did not use primer set on this sample, 1 = Used primer set on this sample)
IDididNumberA sequence of unique integers that identifies each blood smear
SPECIES_NAMEspecies_namespecies_nameTextThe scientific name of the organism
SIZEsizesizeNumberCarapace size (mm). Null = not reported for this specimen
SEXsexsexNumberCrab sex (1 = male, 2 = female, 3 = unknown)
ITS_AMPLIFICATIONits_amplificationits_amplificationNumberAmplification (0 = No DNA amplification using this primer set, 1 = DNA was amplified using primer set, 9 = no amplification to report due to primer set not used in sample)
ITS_PRIMERits_primerits_primerNumberPrimer (0 = Did not use primer set on this sample, 1 = Used primer set on this sample)
COLLECTION_LOCATIONcollection_locationcollection_locationTextGeneral location of collected
SMEAR_COVERSLIPPEDsmear_coverslippedsmear_coverslippedNumberTreatment of blood smears (0 = no cover slip, 1 = cover slip attached using mounting media).
SMEAR_STAINEDsmear_stainedsmear_stainedTextSmear staining treatment
16S_AMPLIFICATION_16s_amplification_16s_amplificationNumberAmplification (0 = No DNA amplification using this primer set, 1 = DNA was amplified using primer set, 9 = no amplification to report due to primer set not used in sample)
18S_PRIMER_18s_primer_18s_primerNumberPrimer (0 = Did not use primer set on this sample, 1 = Used primer set on this sample)
COI_AMPLIFICATIONcoi_amplificationcoi_amplificationNumberAmplification (0 = No DNA amplification using this primer set, 1 = DNA was amplified using primer set, 9 = no amplification to report due to primer set not used in sample)
18S_AMPLIFICATION_18s_amplification_18s_amplificationNumberAmplification (0 = No DNA amplification using this primer set, 1 = DNA was amplified using primer set, 9 = no amplification to report due to primer set not used in sample)
STATISTICALstatisticalstatisticalNumberAnalyzed using two-sample t-test (0 = smear was not used in statistical analysis, 1 = smear was used in statistical analysis)
SMEAR_RATINGsmear_ratingsmear_ratingTextHematodinium parasite presence on smear was identified as T-rating. See metadata for key
SPNOspnospnoNumberSpecimen number
SHELL_CONDshell_condshell_condNumberChionoecetes carapace shell condition (0 = premolt, 1 = soft, 2 = new hardshell, 3 = oldshell, 4 = oldshell, 9 = no shell condition information reported for crab specimen or does not apply)
YEARyearyearNumberYear specimen was collected

Upstream Metadata