Comparison of four bacteriophages exposed to chlorine on stainless steel: complete data
The 2014 West African Ebola virus disease outbreak was the largest to date, and conflicting, chlorine-based surface disinfection protocols to interrupt disease transmission were recommended. We identified only one study documenting surface disinfection efficacy against the Ebola virus, showing a >6.6 log reduction after 5-minute exposure to 0.5% sodium hypochlorite (NaOCl) based on small-scale tests (Cook et al. (2015)). In preparation for future extensive, large-scale disinfection efficacy experiments, we replicated the Cook et al. experiment using four potential BSL-1 surrogates selected based on similarities to the Ebola virus: bacteriophages MS2, M13, Phi6, and PR772. Each bacteriophage was exposed to 0.1% and 0.5% NaOCl for 1, 5, and 10 minutes on stainless steel. MS2 and M13 were only reduced by 3.4 log and 3.5 log after a 10-minute exposure to 0.5% NaOCl, and would be overly conservative surrogates. Conversely, PR772 was too easily inactivated for surrogate use, as it was reduced by >4.8 log after only 1-minute exposure to 0.5% NaOCl. Phi6 was slightly more resistant than the Ebola virus, with 4.1 log reduction after a 5-minute exposure and not detected after a 10-minute exposure to 0.5% NaOCl. We therefore recommend
Phi6 as a surrogate for evaluating the efficacy of chlorine-based surface disinfectants against the Ebola virus.
Querying over HTTP
Splitgraph serves as an HTTP API that lets you run SQL queries directly on
this data to power Web applications. For example:
curl https://data.splitgraph.com/sql/query/ddn \
-H "Content-Type: application/json" \
-d@-<<EOF
{"sql": "
SELECT *
FROM \"usaid-gov/comparison-of-four-bacteriophages-exposed-to-fz8p-6n6k\".\"comparison_of_four_bacteriophages_exposed_to\"
LIMIT 100
"}
EOF
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